Non-contact and non-invasive features make it a powerful tool in the field of germ cell applications
Applications of the four-wave transverse shear interference (FIS4) technique in germ cell detection may include the following:
1. Morphological evaluation of germ cells: FIS4 technique can be used to evaluate morphological characteristics of sperm and egg. For sperm, this includes the shape and size of the head and the structure of the midsection and tail, which are all important indicators of sperm health and function. For eggs, the maturity of the oocyte and the quality of the follicle can be checked.
2. Germ cell motility analysis: With FIS4 technology, it is possible to monitor the motility characteristics of sperm, such as movement speed and trajectory, which is crucial for assessing sperm motility. Meanwhile, for the egg, changes in the zona pellucida (zona pellucida) surrounding it can be observed, which may be related to the viability and fertilization ability of the egg.
3. Fertilization process monitoring: FIS4 technology can be used to observe the fertilization process, including the union of sperm and egg, oocyte changes after fertilization, and the stage of early embryonic development.
4. Embryo development assessment: In artificial assisted reproductive technology, FIS4 technology can be used to monitor the early stages of embryo development, assess its morphological and dynamic changes, and thus help select potential embryos for transfer.
Because germ cells and early embryos are extremely small and fragile, the non-contact and non-invasive nature of the FIS4 technology makes it a powerful tool for these applications.
Measurement advantage
Complete measurement with a single frame
◆ No dyeing required
◆ Live 3D morphology real-time observation
◆ Support counting, segmentation, activity analysis
High resolution
◆ Up to 512×512 phase sampling points
◆ High dynamic range
◆ Nanoscale sensitivity
Easy to adjust integration
◆ Small and compact
◆ Support parallel and convergent beams
◆ Super vibration resistance
Measurement example
▎Germ cell detection light path
▎Detection of ovarian granulosa cell tumor
Measurement demonstration
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